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In vitro and in vivo models to evaluate the impact of engineered nanoparticles on kidney function

project cellesi2

Project overview
This project aims to assess and predict the nanotoxicity of engineered nanoparticles on kidney function, thus unveiling key physical and biological mechanisms of interaction between nanoparticulate, the glomerular filtration barrier, and the tubular and vascular renal compartments. These targets will be achieved by synthesizing a library of polymeric bio-nanomaterials of tuneable size (5-200nm), surface properties (i.e. positively charged, negatively charged, protein-repellent surface) and bio-degradability (using as polymeric constituents polyesters of different kinetics of hydrolysis). The effect of these engineered nanoparticles on renal glomerular and tubular cells will be investigated in vitro using conventional cultured cells and a recently developed three-dimensional co-culture system that constitutes an in vitro model of the glomerular filtration barrier, while in vivo tests will be carried out to characterise mechanisms of particle clearance/agglomeration, and potential renal damage, including vascular insults and/or immune activation and inflammatory cell infiltration

Principal Investigator
Prof. Francesco Cellesi
          
Thematic area
Drug delivery, Theranostics

Duration and dates
tot. 40 months, Jul. 2014 – Oct. 2017

Financing institutions
Fondazione Cariplo

Partner institutions
CEN, Fondazione IRCCS Ca’ Granda Ospedale Maggiore Policlinico, Politecnico di Milano

Project team
Francesco Cellesi, Piergiorgio Messa, Maria Teresa Gandolfo, Simone Vettoretti, Li Min, Riccardo Bruni, Davide Moscatelli, Massimo Morbidelli, Stefano Buzzaccaro, Stefania Ordanini, Matteo Rizzotto, Alberto Cesana, Umberto Palmiero Capasso, Simone Gatti

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